Coding

Part:BBa_K357007:Design

Designed by: Anish Kapadia   Group: iGEM10_Toronto   (2010-07-16)


Fusion of muconolactone D-isomerase (Enzyme 3) and 3-oxoadipate enol-lactonase (Enzyme 4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The selected linker is rigid to prevent the two fusion componenet enzymes from collapsing on each other. At the same time the fusion must display channeling capabilities.


Source

The part is constructed form the genomic sequence from P. Putida, optimized to E. Coli.

References