Coding
Part:BBa_K357007:Design
Designed by: Anish Kapadia Group: iGEM10_Toronto (2010-07-16)
Fusion of muconolactone D-isomerase (Enzyme 3) and 3-oxoadipate enol-lactonase (Enzyme 4)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The selected linker is rigid to prevent the two fusion componenet enzymes from collapsing on each other. At the same time the fusion must display channeling capabilities.
Source
The part is constructed form the genomic sequence from P. Putida, optimized to E. Coli.